Following are our most Frequently Asked Questions:
Where can I find Material Safety Data Sheets (MSDS)?
MSDS files can be viewed here, and are downloadable as pdf files.
How does temperature affect the test results?
The PIPER instrument is designed to function in typical laboratory settings for optimal performance, 15°C ~ 30°C (59°F ~ 86°F).
How do I dispose of extra kit reagents or a used cartridge?
Please refer to the package insert on how to properly dispose of excess and used kit components.
What is MagDrive?
MagDrive™ is the name of our ferrofluid technology, the basis of the PIPER system.
Where can I get further information about PIPER other than the manual that comes with the test kit?
You can visit www.ancera.com for further information on PIPER or you can arrange for a training session by calling our sales team.
Can I order individual kit components, such as the cartridge only?
No. In order to maintain consistency of tests, a kit containing all components must be ordered through our standard process.
What is the limit of detection of PIPER tests?
The limit is 3.6 log10 CFU in the sample well at the start of the run. Below this level, PIPER reports "<3.6"
What kind of samples can I run?
The PIPER system has been validated for Poultry Rinsate and Environmental samples.
Do I need to add 275 µl of sample, or can I add more (or less)?
Use only the indicated volumes in the package insert to obtain correct PIPER counts.
Can I reuse any of the kit components? (e.g. excess reagent?)
No. In order to maintain integrity of the kit's components and expiry dates of the kit, you can not re-use any excess reagents. If you have excess re-agents, please dispose of them based on the disposal method described in the kit package insert.
Do I have to incubate samples for 7 hours? Is there a minimum or maximum sample incubation time?
It is not recommended to incubate outside the indicated range. Refer to the kit package insert for the minimum and maximum incubation times.
What if I don't want to run 12 samples at the same time?
The PIPER cartridge supports up to 12 simultaneous independent tests. You may run fewer than 12 tests at one time if desired; however, the cartridge may only be used once.
Do I have to incubate samples at 42°C?
Yes. The PIPER system has been optimized to produce the most accurate results at 42°C.
Can I run different sample types?
At this time the PIPER system has been validated for Poultry Rinsate and Environmental samples only.
How do I turn PIPER on?
Refer to the user manual on instrument setup for connecting the power cord. Then simply toggle the power switch on the back panel to the 'on' position.
What is included in the kit?
A salmonella enumeration and detection kit contains ferrofluid, labeling reagent, control reagent, labeling reagent buffer, control buffer and a cartridge. For exact details on fill volumes and quantities please refer to the kit package insert.
What type of on-screen message does PIPER display when the test didn’t work?
If PIPER cannot determine a reliable count for a sample, it will report “indeterminate” for that sample.
How often does PIPER need a software update or service check?
There is no fixed interval for updating the software, however you can check for updates on our options page. If any critical updates are necessary you will be contacted by Ancera Customer Support.
Can I use the cartridge and cff (concentrated ferrofluid) with my own buffer?
No. Use only the materials supplied in the kit for running a PIPER assay.
Can I partially use a cartridge, then run the unused lanes at another time?
No. The cartridge can only be used once. Any additional usage may lead to cartridge instability and inaccurate results.
Can I keep the kits at room temperature?
The kit and its components must be stored at 2° to 8° C (35.6° to 46.4° F.)
Can I use a kit that has not been stored at 2° to 8° C?
No. Any kit or its components not stored at 2° to 8° C (35.6° to 46.4° F) would need to be disposed of per the disposal method described in the kit package insert.
Can I use a single channel pipette to aliquot the ferrofluid?
We recommend that you only use a repetitive pipette to accurately dispense Ferrofluid at the indicated volume.
I reconstituted the control with the labeling reagent buffer. Can I proceed with my test?
No. The control should only be diluted with the control buffer. Please refer to the package insert for more detailed instructions.
I diluted the labeling reagent with the control buffer. Can I proceed with my test?
No. The labeling reagent should only be diluted with the labeling reagent buffer. Please refer to the package insert for more detailed instructions.
What is a PIPER count?
The PIPER system uses sophisticated image processing software to determine the number of cells captured in the test window. These are reported as PIPER counts.
What do the results on the result screen mean?
The results are PIPER’s estimate of the number of CFU (log10) in the sample well at the start of the run.
Can results be saved or imported? If so, in what format?
PIPER's results are saved in .csv format.
How do I place an order?
Please call 203-315-9985 or talk to your sales representative.
Can I add the labeling reagent to the cartridge once the assay has been started?
No. Failing to add all reagents to the cartridge prior to the start of the run can lead to inaccurate results.